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Magnesium gates glutamate-activated channels in mouse central neurones

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After washing three times using 1x PTW/0.5% BSA solution for at least 30 min (10-min interval), the dissected gonads were stained with DAPI solution (100 ng/mL) for 10 min at room temperature and were then washed with 1x PTW/0.5% BSA solution three times