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The purified TRF1 TRFH domain was then concentrated by centrifugation on a Vivaspin Turbo 15 centrifugal concentrator (10 kDa MWCO) to either ~ 3 mg/mL for NMR studies or ~ 30 mg/mL for crystallisation before flash-freezing and storage at 80 C (Supplementary Fig

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If reconstituted BPC-157 must be frozen (for example, for long-term storage in a research freezer), it should be divided into single-use aliquots so that each portion is thawed only once