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Then, the sections were coated with an anti-CD31 antibody (Santa Cruz Biotechnology, Dallas, TX) at 4 C overnight, followed by incubation with an HRP-labeled secondary antibody (Gene Tech, Shanghai, China), and the sections were visualized with a DAB system (Gene Tech)

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10.3389/fmed.2023.1184860 14 SaccoR.EggenhoffnerR.GiacomelliL

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Morning total testosterone below 300 ng/dL should be confirmed with a second measurement, and additional testing (LH, prolactin) may be warranted if low testosterone is confirmed