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Inositol in Polycystic Ovary Syndrome: Restoring Fertility through a Pathophysiology-Based Approach

doi: 10.1007/s00125-007-0689-x

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On days 1 4, cells were fixed using 4% paraformaldehyde in PBS for 15 min, washed twice with PBS, and then kept in 200 l of PBS supplemented with 1 mg/mL Hoechst and imaged as previously described 15