These investigations focus on laboratory-based examination of peptide structure confirmation, copper-binding behavior, molecular stability profiling, and signaling pathway analysis under defined analytical conditions
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Notably, 30 g of total protein extracts was loaded and electrophoresed with 8% or 10% SurePAGE gels (GenScript, China) or Color PAGE Gel Rapid Preparation Kit (Epizyme Biotech, China), and then blotted onto a PVDF membrane (Millipore, USA)
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