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Filters were blocked with 1 PBS, 5% ( w / v ) no-fat dried milk (PM) for 40 min at room temperature and then probed with one of the next primary antibodies: anti-TGF- (Santa Cruz Biotechnology, sc-130348), anti-IL-10 (Santa Cruz Biotechnology, sc-8438) or anti-IkB- (Santa Cruz Biotechnology, sc-1643), or anti- NF-kB p-65 (Santa Cruz Biotechnology, sc-8008), or anti-TLR4 (Santa Cruz Biotechnology, sc-293072), anti-MyD88 (Santa Cruz Biotechnology, sc-74532), or anti--SMA (Santa Cruz Biotechnology, sc-53015), or anti-Nrf-2 (Santa Cruz Biotechnology, sc-365949), or anti-HO-1 (Santa Cruz Biotechnology, sc-136960), or NQO-1 (Abcam), or anti-PINK1 (Santa Cruz Biotechnology, sc-517353), or anti-Parkin (Santa Cruz Biotechnology, sc-32282) in 1 PBS, 0.1% Tween-20, 5% w / v no-fat dried milk (PMT) at 4 C overnight

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Plaza-DazJGmez-FernndezAChuecaNTorre-AguilarMGilPerez-NaveroJet alAutism spectrum disorder (ASD) with and without mental regression is associated with changes in the fecal microbiota

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