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Microneedling: Applying GHK-Cu immediately after microneedling can accelerate healing, promote deeper collagen remodeling, and enhance overall results by allowing the peptide to penetrate more effectively

ROS is a known inducer of cell proliferation (73)

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After one hour, coverslips were washed in blocking buffer, and further labeled with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen, Carlsbad, CA) for one hour at RT as above

1:2000 dilution), and then in Cy3-conjugated anti-Guinea pig IgG (Jackson Immunoresearch, 1:200) for 2 h

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