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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat

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(40% of the normal intake) was sufficient to increase hypothalamic NPY and AgRP expression in control mice, whereas GHR ablation in AgRP neurons prevented these effects (Fig

However, gastrointestinal side effects also increase proportionally