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The total running time was 12 min: 01 min, 50% solvent B, 19 min, 5040% solvent B, 910 min, 40% solvent B, 1010.1 min, 4050% solvent B, followed by column re-equilibration with 50% solvent B for 1.9 min

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The glomerulus, a cluster of blood vessels inside the nephrons, function as the actual filter