At-home devices are well-suited because they work daily for 5 to 10 years at low cost
Three-micron sections were prepared and mounted on slides, followed by deparaffinization and immunofluorescent staining according to a standard protocol as follows: For deparaffinization, the paraffin-embedded sections were placed at 60 C for at least 30 min, incubated twice in xylene (5 minutes each), and then transferred into 100% ethanol (2 minutes), 100% ethanol (2 minutes), 90% ethanol (1 minute), 80% ethanol (1 minute), and 70% ethanol (1 minute) at room temperature
S.GhaffariN.OlfatiG.et al (2008)
The use of cis-parinaric acid to measure lipid peroxidation in cardiomyocytes during ischemia and reperfusion
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SNAP/FLAG-hGLP-1R internalisation was calculated by first subtracting non-specific signal of wells treated with Mesna without agonist from all the other wells, then normalising to signal of cells with no Mesna exposure (92)
