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Blind cage coding and automated video analysis (EthoVision, TopScan) are recommended Too low n : prior power analysis, n 8 minimum for rodent behavioural tests No positive control : without memantine, donepezil or scopolamine depending on the paradigm, impossible to judge if the model is functional No dose-response : a single tested dose is insufficient to conclude

If the protein is not detected in early fractions but appears in later fractions, it indicates that the column capacity has been exceeded

We need cysteine