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Real-time RT-PCR reaction conditions for dentinsialophosphoprotein ( DSPP ), dentin matrix protein 1 ( DMP1 ), Runt-related transcription factor 2 ( RUNX2 ), osterix ( OSX ), type I collagen ( COL-I ) and osteocalcin ( OCN ) were: 95 C for 10 minutes

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(Updated 2021 Oct 11)