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The aim of the present study was to determine the reliability and accuracy on genotyping and the detection of mixed GT infections using a NGS assay of a fragment of the NS5B region (Roche Diagnostics, from now Roche-NGS), and to compare these results with those obtained using the Abbott RT-PCR which is based on real-time PCR amplification of two genomic viral regions (nonstructural protein 5B [NS5B] and the 5untranslated region [UTR])

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PMID: 9186261