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Plasmids for all WD40 mutants (WD40 Arg13Ala, Lys15Ala, Arg300Ala, and WD40 Tyr33Ala, Lys17Ala) were generated either using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA) or were synthesized by TOPGENE Technologies (Canada) and were purified as described above
Consequently, low activation prevented proliferation in vitro (Supplementary Fig
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Furthermore, the anti-inflammatory effects of these drugs may contribute to their potential neuroprotective benefits against mood disorders
Of the 983 fragment-soaked crystals screened, 696 full datasets could be collected that were of sufficient quality for automated fragment detection analysis using the Pan-Dataset Density Analysis (PanDDA) algorithm 34
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