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This mechanism creates a sustained feeling of fullness from smaller meal portions and prolongs nutrient absorption

The His tag of the purified protein was removed by thrombin treatment, and the protein was applied to a Superdex 200 column (GE Healthcare, Piscataway, NJ, USA) pre-equilibrated with buffer comprising 50 mM Tris-HCl (pH 8.0) and 150 mM NaCl

[14] Other recent studies have found that vitamin D status is unaffected by metformin use, and one study found no noticeable impact of metformin on vitamin D levels at four and 16 months of use in people with type 2 diabetes

Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation

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