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doi: 10.1056/NEJMoa2204705 177 MindellJ

This becomes problematic because patients become dehydrated from not drinking, which harms kidney function

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cDNA clones of AsGGT1 , AsGGT2 , and AsGGT3 were re-isolated by RT-PCR using KOD plus DNA polymerase (Toyobo, Osaka, Japan) and the following primers: AsGGT1-F (5-TCATATTCTGACGCAGATTCCACAG-3) and AsGGT1-R (5-TGTTCAATCATATTTTGTACAAATAGAC-3) for AsGGT1

However, concomitant administration of loop diuretics with thiazide diuretics, which act on the distal tubules, may result in massive diuresis, hypotension and/or dehydration [105]

Micrographs were processed using the Patch Motion Correction and Patch CTF Estimation jobs, then manually curated to remove micrographs with CTF fit resolution cutoffs greater than 5