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Most evidence supports an activating and proinflammatory role for ROS in immune cells and nonimmune cells, including VEC and VSMC 28,29,30,89,90,92,93

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Cysteine deprivation (CC) leads to mitochondrial membrane potential hyperpolarisation and accumulation of lipid peroxides

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Briefly, an aliquot of cell homogenate was incubated with the labeled substrate DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroaniline), which is cleaved by the caspase-3 enzyme and releases the chromophore pNA