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176 In 1906, after the discovery of secretin, it was first tested if the gut aided the pancreas with disposing of nutrients via stimulation of pancreatic internal secretion by Moore and colleagues, who orally administered porcine small intestinal extract to treat diabetic patients

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4 Necroptosis in CP-induced acute kidney injury (AKI) 4.1 Fundamentals of necroptosis 4.1.1 General overview of necroptosis The term programmed cell death had been commonly used to describe caspase-dependent apoptosis until caspase-independent necrosis was identified (Linkermann et al., 2012)

This approach is often used in tightly controlled experiments where consistency and simplicity are prioritised

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Lenti HeLa Tet-One-Puro cell lines were cultured in DMEM +FBS in the presence of 1 g/mL puromycin