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The immune cells, particularly memory T cells, are trapped in a state of hyperproliferation and chronic activation, generating immense amounts of oxidative waste

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Chong et al., 2019)

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Science 304, 13251328

For recycling, cells were incubated with agonists at 37 C for 15 min, followed by endocytosis arrest at 4 C, surface label removal as above, 30 min incubation at 37 C with 10 M exendin(9-39) to prevent further internalization, and a second round of surface label removal at 4 C