The pellet was washed in MNT (30 mM morpholineethanesulfonic acid, 100 mM NaCl, and 20 mM Tris-HCl, pH 7.4) by another round of at 20,000 g for 1 h at 4 C
This is why meat eaters tend to end up with much more TMAO in their blood than vegetarians.A few people have a genetic defect for making a functional FMO enzyme system that converts TMA to TMAO, and so TMA accumulates in their bodies and produces a strong fishy odor
From the above experiments, total enzymatic rates (actual enzymatic plus non-enzymatic rates) were obtained
doi: 10.1016/S0162-0134(02)00511-1
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