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On day 91, the medium was modified to DMEM/F12 (1:1) supplemented with 1% N2, 1 x NEAAs, 1% antibioticantimycotic, 10% FBS, 100 mM Taurine and 2 mM GlutaMAX, and the concentration of retinoic acid supplementation was reduced to 0.5 M for the remainder of the culture period

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