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Equipment needed Gather everything before starting

Shown is the phagocytosis by THP-1 cells (panel A) of ring-infected and non-infected GR-deficient RBCs from the index patient, brother (individual 2) and sister (individual 3) of the index patient, and from normal GR-sufficient controls

Handle with appropriate laboratory safety measures

Typically, DPBF (10 M) was added into the ZFO, ZFP, and ZFP + H 2 O 2 dispersion (final material concentration 200 g mL 1 , final H 2 O 2 concentration 1 mM), followed by US irradiation (1.0 MHz, 1.5 W cm 2 ) for different durations

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Energy Chemical (Shanghai, China)