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Mouse GLP-1R CHO cells were cultured in Hams F12/DMEM (1:1) with L-glutamine (Invitrogen) supplemented with 10% FBS and 0.8 mg/mL geneticin

however, this effect was relatively greater in Glp1r VSM+/+ mice (Figure 4A)

The Ringers solution used in Ussing chamber experiments contained (in mM): NaCl (120), KCl (3.0), MgCl 2 (0.5), CaCl 2 (1.25), NaHCO 3 (23.0), and D-glucose (10.0), and constantly bubbled with carbogen (95% O 2 /5% CO 2 ), pH 7.4 0.2