The locus coeruleus is involved in opioid addiction development: in mice, hypothalamic hypocretin innervation increases after morphine administration, correlated with an increase in tyrosine hydroxylase expression (the enzyme that catalyzes the rate limiting step in this synthesis of catecholamines)
Sleep , nutrition , alcohol , smoking - these will determine your result more than 1 mg vs 2 mg ever will
Ezaki S, Suzuki K, Kurishima C, Miura M, Weilin W, Hoshi R, et al
The second stage is completed by glutathione synthetase (GS), whose substrate -Glu-Cys undergoes peptide bond condensation with glycine, accompanied by the hydrolysis of ATP to provide the required reaction energy
Every other day
After extended use of opioids, stopping will be difficult