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The residue was resuspended with 25 L of a solution consisting of 90% acetonitrile and 10% 20 mM ammonium formate at pH 3.5 (v/v)

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The heart rate signal is slightly stronger due to the glucagon component, but the overall picture is not alarming for healthy adults

107(6):771781 Endocrine and metabolic roles of insulin-like growth factor signaling in experimental models Sjgren K, Jansson JO, Isaksson OG, Ohlsson C., Minerva Endocrinologica , 2002