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The background subtracted SPR sensorgrams were quantitatively evaluated to determine K D (apparent affinity constant) by using the Biacore 3000 Evaluation Software (GE Healthcare) and the Langmuir 1:1 binding model

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Large blinded placebo-controlled human trials are sparse, and the human studies that do exist are frequently small and conducted on multi-ingredient cosmetic formulations rather than GHK-Cu in isolation