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Markers of microbial dysbiosis, such as shifts in the Firmicutes/Bacteroidetes ratio, reduction in butyrate-producing species, and increased LPS levels, may serve as early indicators of circadian-gut axis dysfunction 260

ROS includes free radicals like superoxide anion (O2) and hydroxyl radical (OH), while RNS includes nitric oxide (NO) and peroxynitrite (ONOO)

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Finally, align interpretations with the manuscripts where, when, how much framework to avoid conflating adaptive signalling with downstream injury (Griendling et al