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Rats in the osteoporotic group showed a significant increase in the serum IL-6 by 1.92-fold compared to the control group

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Normal distribution of data was assessed using histograms, qq-plots and Shapiro Wilk tests for normality

The resulting phosphopeptide identifications were used to build a spectral library, which was then searched in peptide-centric mode against the corresponding DIA raw files using Spectronaut with the PTM site localization algorithm implemented

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