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Glutathionylated roGFP2 C151S , roGFP2 C208S , PfGrx C88S , and PfGrx C32S/C88S were produced by incubation of the reduced enzymes with 5 mM GSSG for 60 min on ice

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Despite these findings of lower choline intake compared to recommendations, the crucial observation was that all cats remained healthy throughout the weight reduction period and did not exhibit any clinical signs of choline deficiency