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ROS and live/dead cell staining TSPCs (5000 cells/cm) were cultured on a plastic petri dish and treated in duplicate with 500 M NAC and/or 500 M hydrogen peroxide (H 2 O 2 ) for 12 h [55, 56]

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GSH deficiency on hepatocyte epigenetic modifying enzymes Hepatocytes treated with GCLC siRNA showed elevated Dnmt, Tet2, and Tet3, but Tet1 was downregulated, and there was no change in Tdg mRNA expression (Fig