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n = 100) or placebo (control group

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5e), but to determine the persistence of agonistreceptor complexes, we adapted our TR-FRET binding assay to include reversible labeling using cleavable SNAP-biotin complexed to streptavidin-terbium to label the surface GLP-1Rs and, after internalization with different FITC-agonists, stripping residual surface receptors of label to ensure that FRET was derived only from internalized receptors (see Fig