They were housed under controlled laboratory conditions with a 12h/12 h light/dark cycle, at a constant temperature of 25C 1C and relative humidity of 60% 10%
Severe global cerebral ischemic event remarkably disturbed the balance among these metabolites in correlation with brain regional dispirited perfusion rate restriction and caused a decrease in the Glu/Gln and GABA/ Gln ratios with the tendency to partial gradual recovery over 72 h reperfusion in all analyzed regions
After reconstitution, aliquot into small volumes to avoid repeated freezethaw cycles
Analyzed indices included bone volume fraction (BV/TV), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th) and tissue mineral density (TMD)
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J Cardiovasc Pharmacol 77:238244 Patel DC, Tewari BP, Chaunsali L, Sontheimer H (2019) Neuronglia interactions in the pathophysiology of epilepsy
