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Despite the fact that our experiments were conducted in mice, the kynurenine pathway, along with neurochemical markers such as GFAP, NSE, and NEFL, may have important implications for understanding neuropsychiatric and metabolic disorders in humans with diabetes and depression

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Tissue preparation for light microscopic immunohistochemistry/immunofluorescence Male C57Bl/6 J mice (n = 3, 1213 weeks old, weighing 2731 g) were anesthetized with a mixture of ketamine and xylazine as described above, and perfused transcardially with 10 ml PBS (pH 7.4), followed by 40 ml 4% paraformaldehyde in 0.1 M PB (pH 7.4)