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Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added

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Injection Technique Subcutaneous injection technique, following established clinical practice and CDC guidance [13] [14]

This is because a standard serum test measures the total amount of B12 in your blood, not the amount that is actively available for your cells to use