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cerevisiae strain was maintained on Y east E xtract P eptone D extrose (YEPD) agar medium and grown in liquid YEPD medium at 30 C with aeration on a shaker

This structural modification protects it from rapid degradation by the enzyme dipeptidyl peptidase-4 (DPP-4), resulting in a half-life of approximately 13 hours, compared to minutes for endogenous GLP-1

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