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ES2, OVCAR3 and OVCAR8 cell lines were cultured in DMEM (41965039, Gibco, Life Technologies) supplemented with 1% FBS (S 0615, Merck), 1% antibiotic-antimycotic (AA) (P0607300, PAN Biotech)

This provides a sustained signal that primes the pituitary for a prolonged period of GH release, deeply supporting long-term physiological regulation

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To clarify the upstream regulatory mechanism of miR-29b-3p under Gln deprivation conditions, we focused on c-Myc as a key candidate transcription factor