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The reaction is based on the reduction of GSSG to GSH with GR in the presence of NADPH leading to the formation of a colored product, i.e., 5-thionitrobenzoate

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Even though ethanol extracts are the most used to produce tablets and other galenic preparations, comparison studies among the various types of preparations, like the essential oil and the oily macerate, have not yet revealed which are most effective and which active constituents are the most important [132]