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Nemunaitis J, Vukelja SJ, Richards D, et al

For them, the cyanide can accumulate and this form of B12 can make things worse

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After four washes for 10 min in Triton X-100- and albumin-supplemented PBS, and one wash in PBS at RT, diamidine-2-phenylindole-dihydrochloride (DAPI, 236276, Roche Diagnostics) was used as fluorescent nuclear stain at 0.1 g/mL in PBS for 10 min