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Briefly, bacteria were removed from the culture at 2000 g for 10 min and the minicells sedimented from the supernatant at 10,000 g for 30 min
Targeting oncometabolism to maximize immunotherapy in Malignant brain tumors
This ensures that what is on the label is actually in the bottle and that the product is free from heavy metals, molds, or other contaminants
Jiang, Y., Han, Y., Qiao, P., & Ren, F
Protoplast preparation (adapted from ref 1) 1