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The Cosmetic Ingredient Review (CIR) Expert Panel has reviewed it and concluded it's safe as used

BMC complementary and alternative medicine, 12, 70

L., Kennedy, D

Heart 2012;98:408413

[DOI] [PMC free article] [PubMed] [Google Scholar] 98.Hashimoto T., Perlot T., Rehman A., Trichereau J., Ishiguro H., Paolino M., Sigl V., Hanada T., Hanada R., Lipinski S., Wild B., Camargo S.M.R., Singer D., Richter A., Kuba K., Fukamizu A., Schreiber S., Clevers H., Verrey F., Rosenstiel P., Penninger J.M

Western blot analysis Cells were washed with PBS containing 1 mmol L 1 N-Ethylmaleimide (NEM, Santa Cruz, #sc-202719), then lysed with Laemmli Sample buffer containing 2 % SDS, 4 % glycerol, 40 mmol L 1 Tris-Cl (pH 6.8), 5 % 2-Mercaptoethanol, 0.01 % bromophenol blue, 1 mmol L 1 NEM, 1 mmol L 1 Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor mixture (Roche, #COEDTAF-RO), and phosphatase inhibitor mixture (Roche, #04906837001)