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Traditionally, in vitro studies of intestinal biology have relied on immortalized cell lines, which, while informative, often fall short in recapitulating the intricate cellular interactions and physiological processes observed in the human intestine (169, 170)

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Longitudinal studies reinforce this perspective, illustrating that poor glycemic control in patients with diabetes can further deteriorate sleep quality, perpetuating a detrimental cycle of worsening health outcomes [53]

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Obesity is a major risk factor for MAFLD, free fatty acids and metabolites released by adipocytes and cytokines secreted by macrophages in adipose tissue contribute to the of development MAFLD (Woo and Lavine, 2016)