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The analgesic effects of liraglutide were not observed in mice with constitutively active glycogen synthase kinase-3 beta (GSK3), as activation of GSK3 promotes the activation of the NLRP3 inflammasome [48]

In contrast, transient interactions between the exendin-P5 N-terminus and TMD are clearly important for agonism, with the majority of residues within this cavity being required for eliciting cAMP signalling (Figures 4-5, Supplemental Figure 10)

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This is slightly longer than the half-life of endogenous GLP-1, which has a half life of about 2 minutes, primarily via degradation by endogenous DPP-4