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Briefly, cells pretreated with 4 mM glutamine (Gln) and 0 mM Gln, respectively, were cross-linked with formaldehyde at 37 C for 10 min, followed by the addition of glycine solution to quench the cross-linking reaction

Collection tubes were inverted 10x to ensure proper mixing of blood and anticoagulant and then placed on wet ice for transportation to the laboratory

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doi: 10.1001/archderm.1977.01640120064011 203 LouchezAEl AarbaouiTWeillAGaudinOJeltiLBelmondoTet al