After washing five times with lysis buffer, the complexes were mixed with lysis buffer containing 290 mM imidazole, and the bound proteins were eluted by boiling in 5 sodium dodecyl sulfate (SDS) loading buffer for 5 min
Vigorously shaking the vial can denature the peptide, meaning its complex folded structure is damaged
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Given that GLP-1 agonists can cause gastroparesis, there is increased concern for ileus when the patient is taking an antipsychotic medication with a high ACB, such as Clozapine, Olanzapine, Quetiapinethe same medications for which we are wanting to manage metabolic side effect consequences
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