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This study was supported by the Shandong University of Traditional Chinese Medicine Doctoral Student Quality Improvement Innovation Project (YJSTZCX2024018), the Inheritance Studio of Traditional Chinese Medicine Master Zhang Canjia of State Administration of Traditional Chinese Medicine (no.(2010) 59), the Chinese Medicine Science and Technology Project of Shandong Province in 2023 (M-2023031), and the Key Project of Undergraduate Teaching Reform Research in Shandong Province (Z2023292)

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Most modified peptides could be detected using both untargeted (DDA) and targeted (MRM) approaches, however the latter yielded better detection sensitivity with higher signal-to-noise and two sites were uniquely found by MRM