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Measures were taken to minimise pain and discomfort of the animals in accordance with the Directive 40/2013 (II.14): On animal experiments issued by the Government of Hungary, and the Directive 2010/63/EU On the protection of animals used for scientific purposes issued by the European Parliament and the European Council

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Within the central nervous system, particularly in appetite-regulating centres of the hypothalamus, semaglutide reduces hunger signals and increases feelings of satiety

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Detecting peptides at all requires specialized mass-spectrometry assays, the kind used in competitive anti-doping programs rather than routine screening