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Beads were washed four times with IP wash buffer (50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.1% (vol/vol) IGEPAL CA-630), then thrice with IP wash buffer lacking IGEPAL CA-630

Different studies have demonstrated that theres a clear connection between high homocysteine levels and cardiovascular diseases (and related mortality)

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Perseus software 62 (version 1.6.1.3) was used for calculation of the significance B (psigB) for each protein ratio with respect to the distance of the median of the distribution of all protein ratios as well as protein intensities 59

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