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Glutathione levels naturally decline because the body depends on them every day to handle stress and maintain balance

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A method for isolating a peptide or GLP-1 peptide, the method including purification of a peptide or GLP-1 peptide from a mixture comprising said peptide or GLP-1 peptide and related impurities via a cation exchange chromatography process, the cation exchange chromatography process comprising the steps of: separating said peptide or GLP-1 peptide and said related impurities of said mixture by elution in a solution comprising an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said peptide or GLP-1 peptide has a positive local or overall net charge different from the local or overall positive net charge of said related impurities so as to remove said related impurities

The combination approach worked best when hydration was restored first, followed by collagen stimulation later

doi: 10.1016/s0092-8674(03)00393-3