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In the HCC ACLY KO cells, we quantified acetyl-CoA in the presence or absence of acetate, finding that acetyl-CoA abundance is elevated in ACLY KO cells in the presence of acetate and decreases back to that in WT cells in its absence (Figure 1F)

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However, this idea was to some extent supported by the finding of somewhat more pronounced phenotypes in double and triple knock-out animals (30)

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