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Next, cells were lysed with lysis buffer (50 mM TrisHCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 10 g/ml aprotinin, 10 g/ml leupeptin, 5 mM PMSF and 1 mM DTT and subsequently centrifuged at 18,000 g for 20 min at 4 C

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